English: RP-HPLC chromatograms of Bergamot Juice at 283 nm (A) and 325 nm (B) - The figure shows the chromatograms obtained for the BJ used in our study and extracted at 283 nm and 325 nm. BJ is characterized by the presence of seven flavanones showing maximum absorption at 283 nm (Fig. 1A) namely eriocitrin ([M-H]- 595 (287)), neoeriocitrin ([M-H]- 595), narirutin ([M-H]- 595 (271)), naringin ([M-H]- 595), neohesperidin ([M-H]- 609), naringin-di-oxalate ([M-H]- 621 (579)) neohesperidin-di-oxalate ([M-H]- 651 (609)), and five flavones that show maximum absorption at 325 nm (Fig. 1B): apigenin-6,8-di-C-glucoside ([M-H]- 593), diosmetin-6,8-di-C-glucoside ([M-H]- 623), neodiosmin ([M-H]- 607), rhoifolin ([M-H]- 577), diosmin ([M-H]- 607 (299)). These qualitative data are in good agreement with those previously reported in literature [2], [3], [4], [5] and confirmed that BJ is rich in flavonoids. Table 1 summarizes the flavonoid content (mg/L ± SEM) of the sample, and also illustrates the maximum absorption wavelength (λmax), parent ion ([M-H]-) and coefficient of variation (CV%) values of each compound in the sample analyzed. Neoeriocitrin, naringin and neohesperidin are the most abundant flavanones in the BJ analyzed, representing with naringin-di-oxalate and neohesperidin-di-oxalate about the 90% of total flavanone-O-glycosides. Our data, concerning the quantitative content of flavanone-β-neohesperidosides of BJ, are in agreement with previous reports [5]. Identification was carried out by combining the information of both UV and MS data with that of reference materials when available.